Docenti

Sandra Zampieri

Sandra Zampieri

Assistant Professor

Telefono: 0498212230

Phone lab: 0498276030

E-mail: sanzamp@unipd.it

Education

1996: Degree in Biological Sciences at the University of Padova
1998: Habilitation in Biology
2002: PhD in Experimental Rheumatology

Positions

September 2019 - present: Assistant Professor of General Pathology, University of Padova, Italy
2013-2019: Senior researcher at the Department of Biomedical Sciences in Padova, University of Padova
2006-2012:  Research Collaborator at the Venetian Institute of Molecular Medicine in Padova, Italy
2002-2006:  Postdoctoral Trainee at the Division of Rheumatology, University of Padova
1999-2000: Visiting Researcher at the Department of Biochemistry, University of Nijmegen, Netherlands

During the PhD, the research activity has addressed the role of apoptosis in the pathogenesis of autoimmune diseases, such as SLE and Inflammatory myopathies. During the post doctoral trainee, the research interest has focused on studies on muscle plasticity and their application to medical research, in particular on the role of physical exercise and electrical stimulation to restore muscle mass. The current research topic concerns the physiopathology of denervation-related skeletal muscle wasting conditions, such as aging and cancer cahexia by morphological characterizations and expression analyses of the signalling pathways that regulate skeletal muscle trophism and innervation.

1. Studies on the role of apoptosis in the pathogenesis of the exercise-induced skeletal muscles damage and in the induction of autoimmune response in SLE.

Zampieri S, Degen W, Ghirardello A, Doria A, van Venrooij WJ. Dephosphorylation of autoantigenic ribosomal P proteins during Fas-L induced apoptosis: a possible trigger for the development of the autoimmune response in patients with systemic lupus erythematosus. Ann Rheum Dis 2001; 60: 72-76.

Podhorska-Okolow M, Sandri M, Zampieri S, Brun B, Rossini K, Carraro U. Apoptosis of myofibres and satellite cells: exercise-induced damage in skeletal muscle of the mouse. Neuropathol Appl Neurobiol 1998; 24: 518-31.

2. Characterization and serological detection of antibodies in autoimmune diseases.

Zampieri S, Ghirardello A, Rossini K, Iaccarino L, Bassi N, Atzeni F, Sarzi-Puttini P, Doria A. Antigen preparation for immunological studies in systemic autoimmune diseases. Ann NY Acad Sci 2007; 1109: 193-202.

Zampieri S, Ghirardello A, Iaccarino L, Tarricone E, Gambari PF, Doria A. Anti-Jo-1 antibodies. Autoimmunity 2005; 38: 73-78.

Zampieri S, Mahler M, Blüthner M, Qiu Z, Malmegrim K, Ghirardello A, Doria A, van Venrooij WJ, Raats JMH. Recombinant anti-P proteins autoantibodies isolated from a human autoimmune library: reactivity, specificity and epitope recognition. Cell Mol Life Sci 2003; 60:588-98.

Zampieri S, Ghirardello A, Doria A, Tonello M, Bendo R, Rossini K, Gambari PF. The use of Tween 20 in immunoblotting assays for the detection of autoantibodies in connective tissue diseases. J Immunol Methods 2000; 239: 1-11.

3. Clinical and histopathological characterization of the paraneoplastic forms of myopathies

Zampieri S, Valente M, Adami N, Biral D, Ghirardello A, Rampudda ME, Vecchiato M, Sarzo G, Corbianco S, Kern H, Carraro U, Bassetto F, Merigliano S, Doria A. Polymyositis, dermatomyositis and malignancy: A further intriguing link. Autoimmun Rev 2010; 9: 449-53.

Zampieri S, Doria A, Adami N, Biral D, Vecchiato M, Savastano S, Corbianco S, Carraro U, Merigliano S. Subclinical myopathy in patients affected with newly diagnosed colorectal cancer at clinical onset of disease: evidence from skeletal muscle biopsies. Neurol Res, 2010, 32: 20-5.

4. Characterization of the structure and function of long term denervated skeletal muscles in patients affected with Conus Cauda lesion and in the animal model of denervated adult rats.

Mancinelli R, Kern H, Fulle S, Carraro U, Zampieri S, La Rovere R, Fanò G, Pietrangelo T. Transcriptional profile of denervated vastus lateralis muscle derived from a patient 8 months after spinal cord injury: a case-report. Int J Immunopathol Pharmacol. 2011; 24: 749-59.

Kern H, Carraro U, Biral D, Adami N, Zampieri S. Severely atrophic muscle fibers with nuclear clumps survive many years in permanently denervated human muscle. The Open Pathology Journal 2009; 3:106-110.

Squecco R, Carraro U, Kern H, Pond A, Adami N, Biral B, Vindigni V, Boncompagni S, Pietrangelo T, Bosco G, Fanò G, Marini M, Abruzzo PM, Germinario E, Danieli-Betto D, Protasi F, Francini F, Zampieri S. A sub-population of rat muscle fibers maintains an assessable excitation-contraction coupling mechanism after long-standing denervation, despite lost contractility J Neuropathol Neurobiol 2009; 68: 1256-68.

5. Studies on the physiopathology of skeletal muscles during denervation, ageing and cancer cachexia: role of physical exercise in the recovery of muscle atrophy

Kern H, Carraro U, Adami N, Biral D, Hofer C, Forstner C, Modlin M, Vogelauer M, Pond A, Boncompagni S, Paolini C, Mayer W, Protasi F, Zampieri S. Home-based Functional Electrical Stimulation (h-bFES) rescues permanently denervated muscles in paraplegic patients with complete lower motor neuron lesion Neurorehab Neural Re 2010; 24:709-21.

Zampieri S, Pietrangelo L, Loefler S, Fruhmann H, Vogelauer M, Burggraf S, Pond A, Grim-Stieger M, Cvecka J, Sedliak M, Tirpáková V, Mayr W, Sarabon N, Rossini K, Barberi L, De Rossi M, Romanello V, Boncompagni S, Musarò A, Sandri M, Protasi F, Carraro U, Kern H. Lifelong Physical Exercise Delays Age-Associated Skeletal Muscle Decline. J Gerontol A Biol Sci Med Sci. 2014 Feb 18.

Mosole S, Carraro U, Kern H, Loefler S, Fruhmann H, Vogelauer M, Burggraf S, Mayr W, Krenn M, Paternostro-Sluga T, Hamar D, Cvecka J, Sedliak M, Tirpakova V, Sarabon N, Musarò A, Sandri M, Protasi F, Nori A, Pond A, Zampieri S. Long-term high-level exercise promotes muscle reinnervation with age. J Neuropathol Exp Neurol. 2014; 73: 284-94.

Kern H, Barberi L, Löfler S, Sbardella S, Burggraf S, Fruhmann H, Carraro U,Mosole S, Sarabon N, Vogelauer M, Mayr W, Krenn M, Cvecka J, Romanello V, Pietrangelo L, Protasi F, Sandri M, Zampieri S, Musaro A. Electrical stimulation counteracts muscle decline in seniors. Front Aging Neurosci. 2014 Jul 24;6:189.

Pigna E, Berardi E, Aulino P, Rizzuto E, Zampieri S, Carraro U, Kern H, Merigliano S, Gruppo M, Mericskay M, Li Z, Rocchi M, Barone R, Macaluso F, Di Felice V, Adamo S, Coletti D, Moresi V. Aerobic Exercise and Pharmacological Treatments Counteract Cachexia by Modulating Autophagy in Colon Cancer. Sci Rep. 2016 May 31;6:26991.

Zampieri S, Mammucari C, Romanello V, Barberi L, Pietrangelo L, Fusella A, Mosole S, Gherardi G, Höfer C, Löfler S, Sarabon N; Cvecka J, Krenn M, Carraro U, Kern H, Protasi F, Musarò A,Sandri M, and Rizzuto R. Physical exercise in aging human skeletal muscle increases Mitochondrial Calcium Uniporter (MCU) expression levels and affects mitochondria dynamics. Physiol Rep 2016, Dec;4(24). pii: e13005.

Tezze C, Romanello V, Desbats MA, Fadini GP, Albiero M, Favaro G, Ciciliot S, Soriano ME, Morbidoni V, Cerqua C, Loefler S, Kern H, Franceschi C, Salvioli S, Conte M, Blaauw B, Zampieri S, Salviati L, Scorrano L, Sandri M. Age-Associated Loss of OPA1 in Muscle Impacts Muscle Mass, Metabolic Homeostasis, Systemic Inflammation, and Epithelial Senescence. Cell Metab. 2017 Jun 6;25(6):1374-1389.e6.

Bolotta A, Filardo G, Abruzzo PM, Astolfi A, De Sanctis P, Di Martino A, Hofer C, Indio V, Kern H, Löfler S, Marcacci M, Zampieri S, Marini M, Zucchini C. Skeletal Muscle Gene Expression in Long-Term Endurance and Resistance Trained Elderly. Int J Mol Sci. 2020 Jun 2;21(11): E3988.

For a complete list of PubMed publications see
https://www.ncbi.nlm.nih.gov/myncbi/sandra.zampieri.1/bibliography/public/